Lipolytic activity of bovine growth hormone bound to sepharose beads
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منابع مشابه
Coupling of glycosaminoglycans to agarose beads (sepharose 4B).
1. Heparin, heparan sulphate, chondroitin sulphate and dermatan sulphate were covalently attached to beads of agarose activated by cyanogen bromide. The bond is probably mediated by the amino group of a serine or peptide residue at the reducing end of the polysaccharide chain. 2. The uptake of glycosaminoglycan during the coupling procedure is about 0.9mg/ml of wet gel. However, direct analysis...
متن کاملMediation of the hepatic effects of growth hormone by its lipolytic activity.
The aim of the study was to investigate the acute effect of GH per se, independent from its lipolytic activity, on glucose and lipid oxidation and glucose turnover in seven healthy subjects. Five tests lasting 360 min were performed. Each test consisted of a 4-h equilibration period followed by a euglycemic hyperinsulinemic (25 mU/kg x h) clamp lasting 2 h. In test 1 (control experiment) saline...
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The lipolytic activity of bovine thyroid-stimulating hormone and its subunits have been compared to the lipolytic activity of ovine-luteinizing hormone and its subunits. When tested on white adipocytes isolated from epididymal fat pads of BALB/c mice, thyroid-stimulating hormone was a potent lipolytic agent, active at a concentration of 7 X lo-lo M. In contrast, the isolated subunits of thyroid...
متن کاملMonoclonal antibodies against bovine growth hormone.
A number of mouse x mouse hybridomas producing monoclonal antibodies (MAbs) against bovine growth hormone (bGH) were prepared by fusion of spleen cells from bGH-primed mice (Balb/c) with non-secretory mouse myeloma cells (PAIOP3) and characterized. MAbs obtained from three fusion experiments belonged to IgM, IgG1 and IgG2b class/subclass of antibodies. Cross-reaction studies indicated that gene...
متن کاملStructural Studies on Bovine Growth Hormone
Purified bovine growth hormone, consisting of a single polypeptide chain, has been found to contain NHz-terminal alanine, phenylalanine, and methionine in nearly equal amounts. The minimum molecular weight calculated from its amino acid composition is 20,846. Five unique fragments have been prepared from bovine growth hormone by cleavage at 4 methionyl residues with cyanogen bromide. In the ord...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1972
ISSN: 0014-5793
DOI: 10.1016/0014-5793(72)80022-x